Incucyte 2021a Download: How to Install and Use the Latest Live-Cell Analysis Software
If you are looking for a powerful and versatile system for live-cell imaging and analysis, you may have heard of Incucyte, a product line from Sartorius that enables you to capture cellular changes in real time inside your incubator. With Incucyte, you can perform a wide range of applications, such as cell health, proliferation, migration, invasion, immune cell killing, neuronal activity, angiogenesis, and more.
But did you know that there is a new version of Incucyte software available? It is called Incucyte 2021a, and it offers many new features and benefits that will make your live-cell imaging and analysis easier, faster, and more insightful than ever before.
incucyte 2021a download
In this article, we will show you how to download and install Incucyte 2021a software, how to set up and run experiments with it, how to visualize and share your results with it, and answer some frequently asked questions about it. By the end of this article, you will be ready to take your live-cell imaging and analysis to the next level with Incucyte 2021a software.
How to Download and Install Incucyte 2021a Software
The first step to use Incucyte 2021a software is to download it from the Sartorius website. You will need the appropriate Incucyte GUI (graphical user interface) software version for your Incucyte instrument. You can find the download link here:
Once you have downloaded the software, you will need to install it on your computer. The installation process is simple and straightforward. Just follow the instructions on the screen and accept the license agreement. You will need to enter the IP address or hostname of your Incucyte instrument to connect.
After installing the software, you will be able to access it from your desktop or start menu. You will see a new user interface that is intuitive and user-friendly. You will also notice some new features that are available with Incucyte 2021a software, such as AI-driven analysis, multi-objective scheduling, multi-user support, remote network capability, expanded vessel compatibility, enhanced graphing tools, metric overlays, data export/import options, and more.
How to How to Set Up and Run Experiments with Incucyte 2021a Software
Once you have installed and connected Incucyte 2021a software to your Incucyte instrument, you are ready to set up and run your experiments. Incucyte 2021a software makes it easy and convenient to design, execute, and monitor your live-cell experiments with a guided interface and a flexible scheduling tool.
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To set up your experiments, you will need to select the type of vessel, the number of wells, the scan frequency, the objective lens, the channels, and the application that you want to use. Incucyte 2021a software supports a wide range of vessels, including 6-, 12-, 24-, 48-, and 96-well plates, as well as slides, flasks, and dishes. You can also choose from different applications, such as cell health, proliferation, apoptosis, cytotoxicity, migration, invasion, immune cell killing, angiogenesis, neuronal activity, reporter gene expression, and more.
After setting up your experiment parameters, you can load your vessel into the Incucyte instrument and start scanning. You can use the scheduling tool to adjust the scan frequency and duration of your experiment at any time. You can also add or remove vessels from the instrument without interrupting the ongoing scans.
To monitor your experiments, you can use the Incucyte VesselView to view and navigate through the images and time courses of your wells. You can zoom in and out of the images, play back the movies, and compare different wells or time points. You can also annotate your images with notes or markers to highlight important events or observations.
How to Use the AI-driven or Classic Analysis Tools to Process and Quantify Your Images with Accuracy and Reproducibility
One of the most powerful features of Incucyte 2021a software is its ability to process and quantify your images with accuracy and reproducibility. You can choose from two types of analysis tools: AI-driven or Classic.
The AI-driven analysis tool uses artificial intelligence algorithms to automatically detect and measure objects of interest in your images. It is fast, accurate, and adaptable to different applications and cell types. You can use the AI-driven analysis tool for applications such as cell health, proliferation, apoptosis, cytotoxicity, migration, invasion, immune cell killing, angiogenesis, neuronal activity, reporter gene expression, and more.
The Classic analysis tool uses predefined masks and thresholds to segment and quantify objects of interest in your images. It is flexible, customizable, and compatible with older versions of Incucyte software. You can use the Classic analysis tool for applications such as cell health, proliferation, apoptosis, cytotoxicity, migration, invasion, immune cell killing, angiogenesis, neuronal activity, reporter gene expression, and more.
Both types of analysis tools allow you to define regions of interest (ROIs), adjust settings and parameters, preview results in real time, apply corrections or filters, and generate metrics for each well or ROI. You can also perform cell-by-cell analysis to obtain single-cell data for each object in your images. How to Visualize and Share Your Results with Incucyte 2021a Software
After processing and quantifying your images, you can visualize and share your results with Incucyte 2021a software. Incucyte 2021a software provides you with various tools and options to create publication-ready graphs and plots, access and share your data with your research team, and export and import your data, images, and movies.
To create graphs and plots, you can use the graphing tools in Incucyte 2021a software to select the metrics, wells, ROIs, and time points that you want to display. You can also customize the appearance of your graphs and plots by changing the colors, symbols, labels, axes, scales, legends, titles, and more. You can also use the metric overlays to add numerical values or statistics to your images or movies.
To access and share your data with your research team, you can use the remote network capability and unlimited user licenses that come with Incucyte 2021a software. You can connect to your Incucyte instrument from any computer on the same network and view or analyze your data. You can also create multiple user accounts with different access levels and permissions for your research team members.
To export and import your data, images, and movies, you can use the data export/import options in Incucyte 2021a software. You can export your data in various formats, such as CSV, XLSX, PDF, PNG, JPG, AVI, or MOV. You can also import your data from other sources or older versions of Incucyte software.
Conclusion
In conclusion, Incucyte 2021a software is a powerful and versatile system for live-cell imaging and analysis that offers many new features and benefits that will make your research easier, faster, and more insightful than ever before. With Incucyte 2021a software, you can:
Download and install the software easily and quickly
Set up and run experiments with a guided interface and a flexible scheduling tool
Monitor your experiments with the Incucyte VesselView
Process and quantify your images with AI-driven or Classic analysis tools
Visualize and share your results with graphing tools, metric overlays, remote network capability, unlimited user licenses, data export/import options
If you are interested in using Incucyte 2021a software for your live-cell imaging and analysis applications, you can request a quote or contact Sartorius for more information here:
You can also find some examples of applications and publications that use Incucyte 2021a software here:
FAQs
What are the system requirements for Incucyte 2021a software?
The system requirements for Incucyte 2021a software are:
Operating System
Processor
Memory
Disk Space
Display Resolution
Windows 10 (64-bit)
Intel Core i5 or higher
8 GB RAM or higher
500 GB HDD or higher
1920 x 1080 or higher
How can I upgrade from an older version of Incucyte software to Incucyte 2021a software?
If you have an older version of Incucyte software (such as Incucyte ZOOM or Incucyte Live-Cell Analysis System), you can upgrade to Incucyte 2021a software by contacting Sartorius. You will need to purchase a new license key for Incucyte 2021a software. You will also need to update the firmware of your Incucyte instrument to be compatible with Incucyte 2021a software.
What are the differences between Incucyte SX5, S3, and SX1 instruments?
The differences between Incucyte SX5, S3, and SX1 instruments are:
Instrument
Vessel Capacity
Lens Options
Channel Options
Spatial Resolution
Temporal Resolution
Data Storage Capacity
Data Transfer Speed
Incucyte SX5
Up to 6 vessels (plates/slides/flasks/dishes)
4x/10x/20x/ 40x
Phase/Red/Green/Blue/NIR
0.8 µm (4x), 0.4 µm (10x), 0.2 µm (20x), 0.1 µm (40x)
As fast as 15 minutes for a 96-well plate
Up to 12 TB
Up to 10 Gbps
Incucyte S3
Up to 6 vessels (plates/slides/flasks/dishes)
4x/10x/20x
Phase/Red/Green
1.25 µm (4x), 0.63 µm (10x), 0.31 µm (20x)
As fast as 15 minutes for a 96-well plate
Up to 4 TB
Up to 1 Gbps
Incucyte SX1
1 vessel (plate/slide/flask/dish)
10x/20x
Phase/Red/Green
0.63 µm (10x), 0.31 µm (20x)
As fast as 15 minutes for a 96-well plate
Up to 2 TB
Up to 1 Gbps
What are the advantages of using AI-driven analysis over Classic analysis?
The advantages of using AI-driven analysis over Classic analysis are:
AI-driven analysis is faster and more accurate than Classic analysis, as it uses artificial intelligence algorithms to automatically detect and measure objects of interest in your images.
AI-driven analysis is more adaptable and robust than Classic analysis, as it can handle different applications, cell types, image quality, and experimental conditions.
AI-driven analysis is easier and more convenient than Classic analysis, as it does not require manual adjustments of masks and thresholds, and provides real-time feedback and results.
What are some of the reagents, kits, and consumables that are compatible with Incucyte 2021a software?
Some of the reagents, kits, and consumables that are compatible with Incucyte 2021a software are:
Incucyte Cell-by-Cell Analysis Kit: This kit enables you to perform single-cell analysis for applications such as cell health, proliferation, apoptosis, cytotoxicity, migration, invasion, immune cell killing, angiogenesis, neuronal activity, reporter gene expression, and more.
Incucyte Immune Cell Killing Assay Kit: This kit enables you to measure the killing activity of immune cells against target cells in real time.
Incucyte Annexin V Red or Green Reagent: This reagent enables you to measure apoptosis in live cells in real time.
Incucyte Caspase-3/7 Green or Red Reagent: This reagent enables you to measure caspase-3/7 activity in live cells in real time.
Incucyte Cytotox Red or Green Reagent: This reagent enables you to measure cytotoxicity in live cells in real time.
Incucyte NucLight Rapid Red or Green Reagent: This reagent enables you to label nuclei in live cells in real time.
Incucyte FabFluor-488 or -568 Secondary Antibody Labeling Kits: These kits enable you to label primary antibodies with fluorescent dyes in live cells in real time.
Incucyte ClearView or OptiView Cell Culture Vessels: These vessels enable you to perform high-quality live-cell imaging and analysis with minimal background and optimal optical clarity.
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